Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: Chondrocyte gene network of CSGalNAcT-1 was classified as glycan biosynthesis and metabolism and functional pathway

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Functional Assay

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: CSGalNAcT-1 and Hapln-1 mRNA expression in osteoarthritis (OA) cartilage, Kashin-Beck disease (KBD) cartilage and normal cartilage. CSGalNAcT-1 and Hapln-1 mRNA levels reach statistical significance. * Denotes significant difference at P < 0.05

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: Comparison of CSGalNAcT-1 expression in the cartilage from osteoarthritis (OA), Kashin-Beck disease (KBD) and normal groups. a , b , c Denote the upper of normal, OA and KBD cartilage, respectively. d , e , f Denote the middle of normal, OA and KBD cartilage, respectively. g , h , i Denote the deep of normal, OA and KBD cartilage, respectively

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: CSGalNAcT-1 and Hapln-1 protein level expression reduced in osteoarthritis (OA) and Kashin-Beck disease (KBD) cartilage compared with normal controls. Wnt 3a, β-catenin and Runx-2 level expression increased in OA and KBD cartilage compared with normal controls. A KBD free-Se group. B 0.25 μg/ml Se + KBD group. C 0.10 μg/ml Se + KBD group. D 0.05 μg/ml Se + KBD group

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing